Fusion polynucleotide for biosynthesis of beta-carotene using bicistronic gene expression and method for producing beta-carotene using the same
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United States of America Patent
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Oct 15, 2013
Grant Date -
Nov 25, 2010
app pub date -
Aug 29, 2008
filing date -
Aug 30, 2007
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Abstract
The present invention relates to fusion polynucleotides for biosynthesis of beta-carotene and a method for producing beta-carotene using the same. More particularly, it relates to fusion polynucleotides encoding phytoene synthase, connective sequences of FMDV-derived 2A sequence or internal ribosome entry site (IRES) and carotene desaturase, and a method for producing beta-carotenes using the same. Fusion polynucleotides and the recombinant vector using the same of the present invention have the effect of expression both phytoene synthase gene and carotene desaturase gene stably within cell transformants. Accordingly, fusion polynucleotides of the present invention can be used to regulate the biosynthetic metabolism of plant producing beta-carotene. Furthermore, it can be applied to effectively increase the content of beta-carotene, a useful metabolite.
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