Method of nucleic acid amplification and measuring reagent and reagent kit therefor
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United States of America Patent
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Oct 15, 2013
Grant Date -
Sep 16, 2010
app pub date -
Oct 17, 2008
filing date -
Oct 19, 2007
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Abstract
The object of the present invention is to provide a method that allows stable amplification of an internal standard material while maintaining an accurate assay value for a target nucleic acid in a nucleic acid detection system involving the use of an internal standard material and a reagent kit used therefor. The present invention relates to a method for nucleic acid amplification comprising preventing an internal standard amplification product from affecting amplification reaction of a target nucleic acid by performing amplification of an internal standard material prior to amplification of the target nucleic acid in the method for amplifying a target nucleic acid in a sample using an internal standard material and a reagent and reagent kit used therefor.
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