Split DNA enzyme for visual single nucleotide polymorphism typing
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United States of America Patent
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Oct 8, 2013
Grant Date -
May 31, 2012
app pub date -
Nov 20, 2009
filing date -
Nov 21, 2008
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Abstract
A probe that changes solution color in the presence of only one of the two DNA sequences, which differ by a single nucleotide, is reported. The probe consists of two oligodeoxyribonucleotides, which form a hydrogen peroxidase-like DNA enzyme when hybridized to the abutting fragments of the complementary analyte. The active peroxidase catalyses oxidation of colorless substrates to the colored products. The probe allows visual detection of a mutation in Alzheimer's disease-related DNA.
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