Composition for use as a peptide retention standard and a method of predicting peptide hydrophobicity in liquid chromatography
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United States of America Patent
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Aug 6, 2013
Grant Date -
Sep 15, 2011
app pub date -
Nov 30, 2009
filing date -
Nov 28, 2008
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Abstract
A composition comprising synthetic peptides for use as a peptide retention standard in liquid chromatography applications is disclosed Said synthetic peptides have a broad range of hydrophobicity while maintaining low variation in their molecular weights The synthetic peptides disclosed consist of the ammo acid sequences LGGGGGGDGSR, LGGGGGGDFR, LLGGGGDFG, LLLGGDFR, LLLLDFR, LLLLLDFR A method of predicting the hydrophobicity of an unknown peptide in liquid chromatography using a composition of peptide retention standards and a method of developing a Universal Hydrophobicity Index (UHI) for the measurement of peptide hydrophobicity in liquid chromatography are also described.
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