Methods and compositions for concentrating secreted recombinant protein

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United States of America Patent

PATENT NO 8486660
APP PUB NO 20120107877A1
SERIAL NO

13346258

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Abstract

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Methods and compositions are described that relate to obtaining concentrated preparations of secreted recombinant proteins. These proteins are expressed in the form of fusion proteins with a chitin-binding domain (CBD). The fusion proteins are capable of being concentrated in the presence of chitin. Also described is: a shuttle vector that includes a modified LAC4 promoter; a chitinase-negative host cell; a CBD capable of eluting from chitin under non-denaturing conditions; and sterilized chitin, which can be optionally magnetized for facilitating recovery of recombinant protein.

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Patent Owner(s)

Patent OwnerAddress
NEW ENGLAND BIOLABS INCMASSACHUSETTS MASSACHUSETTS

International Classification(s)

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  • 2012 Application Filing Year
  • C12P Class
  • 1680 Applications Filed
  • 1225 Patents Issued To-Date
  • 72.92 % Issued To-Date
Click to zoom InYear of Issuance% of Matters IssuedCumulative IssuancesYearly Issuances201220132014201520162017201820192020202120222023202420250255075100

Inventor(s)

Inventor Name Address # of filed Patents Total Citations
Colussi, Paul A Gloucester, US 14 55
Taron, Christopher H Essex, US 31 98

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11.5 Year Payment $7400.00 $3700.00 $1850.00 Jan 16, 2025