Modified polymerases for improved incorporation of nucleotide analogues
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United States of America Patent
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Jun 11, 2013
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Oct 26, 2006
app pub date -
Sep 10, 2004
filing date -
Sep 11, 2003
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Abstract
The invention relates to modified polymerase enzymes which exhibit improved incorporation of nucleotide analogues bearing substituents at the 3′ position of the sugar moiety that are larger in size than the naturally occurring 3′ hydroxyl group. Also described are methods of using the polymerases to incorporate nucleotides into polynucleotides, particularly in the context of DNA sequencing.
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