Multiple-photon excitation light sheet illumination microscope
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United States of America Patent
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May 14, 2013
Grant Date -
May 26, 2011
app pub date -
Oct 29, 2010
filing date -
Oct 29, 2009
priority date (Note) -
In Force
status (Latency Note)
Importance
Abstract
An apparatus for and method of performing multi-photon light sheet microscopy (MP-LISH), combining multi-photon excited fluorescence with the orthogonal illumination of light sheet microscopy are provided. With live imaging of whole Drosophila and zebrafish embryos, the high performance of MP-LISH compared to current state-of-the-art imaging techniques in maintaining good signal and high spatial resolution deep inside biological tissues (two times deeper than one-photon light sheet microscopy), in acquisition speed (more than one order of magnitude faster than conventional two-photon laser scanning microscopy), and in low phototoxicity are demonstrated. The inherent multi-modality of this new imaging technique is also demonstrated second harmonic generation light sheet microscopy to detect collagen in mouse tail tissue. Together, these properties create the potential for a wide range of applications for MP-LISH in 4D imaging of live biological systems.
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International Classification(s)
- H01J:ELECTRIC DISCHARGE TUBES OR DISCHARGE LAMPS
- F21V:FUNCTIONAL FEATURES OR DETAILS OF LIGHTING DEVICES....
- G01J:MEASUREMENT OF INTENSITY, VELOCITY, SPECTRAL CONTE....
- G21H:OBTAINING ENERGY FROM RADIOACTIVE SOURCES; APPLICA....
- G21K:TECHNIQUES FOR HANDLING PARTICLES OR ELECTROMAGNET....
- G01T:MEASUREMENT OF NUCLEAR OR X-RADIATION
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Patent Citation Ranking
- 19 Citation Count
- H01J Class
- 67.73 % this patent is cited more than
- 12 Age
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| Fee | Large entity fee | small entity fee | micro entity fee | due date |
|---|---|---|---|---|
| 11.5 Year Payment | $7400.00 | $3700.00 | $1850.00 | Nov 14, 2024 |
