Isothermal strand displacement amplification using primers containing a non-regular base
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United States of America Patent
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Apr 30, 2013
Grant Date -
Sep 2, 2010
app pub date -
May 25, 2006
filing date -
May 26, 2005
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Abstract
The invention is directed to a method for isothermal DNA amplification comprising providing to the DNA to be amplified an amplification mix comprising a first primer at least partially complementary to a region of DNA and containing a non-regular base, a second primer at least partially complementary to a region of DNA and containing a non- regular base, a DNA polymerase, an enzyme capable of strand displacement, an enzyme that recognises a non-regular base in double-stranded DNA and causes a nick or excises a base in one DNA strand at or near the non-regular base; and amplifying the DNA substantially without thermal cycling.
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