Screening method for identifying PUFA PKS in samples
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United States of America Patent
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Apr 2, 2013
Grant Date -
Nov 8, 2007
app pub date -
Apr 8, 2005
filing date -
Apr 8, 2004
priority date (Note) -
Expired
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Abstract
The invention relates to a method for the rapid and simple identification of PUFA PKS in microorganisms. Said method is characterized by the fact that DNA sections representing PKS that produce specifically for PUFA (polyunsaturated fatty acids) are reproduced in vitro. The PUFA PKS-specific amino acid sequence LGIDSIKRVEIL makes it possible to derive oligonucleotides which are used for the efficient PCR screening for PUFA PKS genes or PUFA-producing microorganisms. The inventive method is particularly suitable for the high throughput screening of microorganisms for PUFA PKS genes.
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