Assay device comprising serial reaction zones
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United States of America Patent
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Apr 2, 2013
Grant Date -
Jan 6, 2011
app pub date -
Jul 1, 2010
filing date -
Jul 2, 2009
priority date (Note) -
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Abstract
An analysis device having at least one sample addition zone, at least one sink, and at least one flow path connecting the at least one sample addition zone and the at least one sink. The at least one flow path includes projections substantially vertical to the surface of the substrate and having a height (H), diameter (D) and reciprocal spacing (t1, t2) such that lateral capillary flow of a liquid sample is achieved. The device includes at least two reaction zones in series, wherein each reaction zone is adapted to facilitating measurement of a response originating from one and the same analyte, and wherein the reaction zones are positioned to allow calculation of the concentration of at least one analyte. Advantages include that a more accurate value can be calculated, variations are reduced, and an estimation of the uncertainty of the response can be calculated.
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