Metabolically enhanced photoautotrophic ethanol producing host cells, method for producing the host cells, constructs for the transformation of the host cells, and method of producing ethanol using the host cells
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United States of America Patent
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Mar 26, 2013
Grant Date -
Jun 7, 2012
app pub date -
Feb 13, 2012
filing date -
Aug 13, 2009
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Abstract
One embodiment of the invention provides a metabolically enhanced photoautotrophic, ethanol producing host cell comprising:
- at least two first metabolic enhancements reducing the enzymatic activity or affinity of at least two endogenous host cell enzymes involved in acetate and lactate fermentation,the first metabolic enhancements resulting in an enhanced level of biosynthesis of acetaldehyde, pyruvate, acetyl-CoA or precursors thereof compared to the respective wild type host cell,at least one second metabolic enhancement different from the first metabolic enhancement comprising an overexpressed enzyme for the formation of ethanol.
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