Process for preparing glycopeptides having asparagine-linked oligosaccharides, and the glycopeptides

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United States of America Patent

PATENT NO 8399656
APP PUB NO 20120035345A1
SERIAL NO

13273786

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Abstract

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Glycopeptide having at least one asparagine-linked oligosaccharide at a desired position of the peptide chain obtained by:

    (1) esterifying hydroxyl of a resin and carboxyl of an amino acid having amino group nitrogen protected with a fat-soluble protective group (AGFPG),(2) removing the protective group to form a free amino group,(3) amidating the free amino group and carboxyl of an amino acid having AGFPG,(4) removing the protective group,(5) repeating the steps (3) and (4),(6) amidating the free amino group and carboxyl of the asparagine portion of an asparagine-linked oligosaccharide having AGFPG,(7) removing the protective group,(8) amidating the free amino group and carboxyl of an amino acid having AGFPG,(9) repeating steps (7) and (8),(10) removing the protective group, and(11) cutting off the resin with an acid;glycopeptide obtained by transferring sialic acid or a derivative thereof to the above glycopeptide.

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Patent Owner(s)

Patent OwnerAddress
GLYTECH INC134 CHUDOJI-MINAMIMACHI SHIMOGYO-KU KYOTO-SHI KYOTO 600-8813

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Inventor(s)

Inventor Name Address # of filed Patents Total Citations
Kajihara, Yasuhiro Toyonaka, JP 39 125

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