A purified vitamin B6-phosphate phosphatase (VB6PP), having the following physico-chemical properties: a) Molecular weight: 29,000±5,000 (consisting of a monomer having a molecular weight of 29,000±5,000); b) Co-factor: Mn2+, Mg2+, Co2+, Sn2+, or Ni2+; c) Substrate specificity: active on pyridoxol 5′-phosphate, pyridoxal 5′-phosphate and pyridoxamine 5′-phosphate; d) Optimum temperature: 30-400° C. at pH 7.5; and e) Optimum pH: 7.0-8.0 is disclosed. Also disclosed are a process for producing VB6PP from a microorganism belonging to the genus Sinorhizobium capable of producing VB6PP, a process for producing vitamin B6 from vitamin B6-phosphate (VB6P) utilizing VB6PP, and a cell-free extract of a microorganism belonging to the genus Sinorhizobium capable of producing VB6PP.

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