Method for detecting or determining abnormal prion protein associated with transmissible spongiform encephalopathy in blood-derived specimen or body fluid-derived specimen
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United States of America Patent
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Feb 5, 2013
Grant Date -
Mar 3, 2011
app pub date -
Aug 22, 2008
filing date -
Aug 23, 2007
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Expired
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Abstract
A pretreatment method of a specimen used for detecting or determining abnormal prion protein (PrPres) associated with transmissible spongiform encephalopathy (TSE), wherein
- (1) a specimen which had been treated with proteinase K is heated in the presence of sodium dodecyl sulfate (SDS) to dissolve proteins and inactivate infectious activity in the specimen at the same time; (2) the specimen processed in the above (1) is cooled under a neutral condition to make abnormal prion protein (PrPres) associated with transmissible spongiform encephalopathy (TSE) aggregated; (3) the aggregate formed in the above (2) is separated from a solution; and (4) the separated PrPres aggregate is detected by the ultrasensitive chemiluminescence method.
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