Immunoassay of analytes in samples containing endogenous anti-analyte antibodies
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United States of America Patent
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Jan 22, 2013
Grant Date -
Oct 1, 2009
app pub date -
Apr 24, 2009
filing date -
Oct 26, 2006
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Abstract
The disclosure provides among other things an assay method that compensates for the presence of endogenous antibodies, e.g., autoantibodies, which might otherwise compromise the measurement of an analyte in a biological sample. In one embodiment, this method entails the use of a two labeled entities: a labeled detection agent and a labeled species-specific antibody, wherein the labeled species-specific antibody is specific for the species from which the biological sample was obtained. Sample analyte is bound by the detection agent and any anti-analyte autoantibodies present in the sample. Analyte bound by autoantibodies is detected via the species-specific antibody, optionally labeled.
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