Method for combined parallel agent delivery and electroporation for cell structures an use thereof
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United States of America Patent
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Dec 25, 2012
Grant Date -
Apr 9, 2009
app pub date -
Jun 9, 2008
filing date -
Nov 6, 1997
priority date (Note) -
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Abstract
Disclosed is a method for parallel delivery of agents to and/or into a cell structure, wherein at least two electrolyte-filled tubes are provided together with a counter electrode, the tubes being connected to a voltage or current generator, said agents being introduced into the electrolyte solution contained in the tubes, which are placed close to the cell structure, whereupon the agents are transported through the tubes to said cell structure and into the said structure through pores which have been formed by application of an electric field focused on the cell structure, resulting in electroporation of the cell structure. Also different applications of the method is disclosed, e.g. use of the method in order to transfer cell-impermeant solutes, such as drugs or genes, into the cell structure or out of the cell structure.
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