Method for the quantitative determination of the concentration of fluorophores of a substance in a sample and apparatus for carrying out the same
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United States of America Patent
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Dec 18, 2012
Grant Date -
May 13, 2010
app pub date -
Nov 12, 2009
filing date -
Nov 13, 2008
priority date (Note) -
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Abstract
The invention concerns methods and apparatuses for quantitatively determining the concentration of fluorophores of a substance in a sample. A constant portion of the reference light of a reference light wave length (λr) emitted by a reference light source is coupled in by an optical element in the direction of a receiving element. A first value corresponding to the portion of the reference light coupled in which is incident on the receiving element is detected. The sample is irradiated with the excitation light of an excitation wave length (λex) emitted by an excitation light source. A second value corresponding to the portion of the fluorescent light of an emission wave length (λem) emitted by the sample which is incident on the receiving element. The ratio of the second value to the first value is determined. The number of fluorophores in the substance is determined based on the ratio.
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International Classification(s)
- H01J:ELECTRIC DISCHARGE TUBES OR DISCHARGE LAMPS
- F21V:FUNCTIONAL FEATURES OR DETAILS OF LIGHTING DEVICES....
- G01J:MEASUREMENT OF INTENSITY, VELOCITY, SPECTRAL CONTE....
- G21H:OBTAINING ENERGY FROM RADIOACTIVE SOURCES; APPLICA....
- G21K:TECHNIQUES FOR HANDLING PARTICLES OR ELECTROMAGNET....
- G01T:MEASUREMENT OF NUCLEAR OR X-RADIATION
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