Nucleic acid hybridization methods
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United States of America Patent
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Nov 27, 2012
Grant Date -
Sep 10, 2009
app pub date -
May 11, 2009
filing date -
Nov 12, 2003
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Abstract
Disclosed herein are methods of destabilizing double-stranded nucleic acid hybridization using an enzyme comprising DNA N-glycosylase activity. Also disclosed herein is the detection of a double-stranded target DNA wherein the hybridization of duplex strands has been at least partially disrupted thereby permitting invasion of a probe strand. Also disclosed herein are methods of using an enzyme comprising DNA N-glycosylase activity to generate single-stranded circular nucleic acids.
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