Detecting molecules
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United States of America Patent
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Oct 30, 2012
Grant Date -
Aug 16, 2007
app pub date -
Aug 20, 2004
filing date -
Aug 21, 2003
priority date (Note) -
Expired
status (Latency Note)
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Abstract
Disclosed are processes to increase the relative abundance of a molecule that is present in a test sample at relatively low abundance. The process includes fractionating a sample and immunizing hosts with the fractions. The antibodies produced from the fractionated test sample are directed primarily to molecules that are in relatively high abundance in the test sample fractions. These antibodies are then contacted with the test sample or a test sample to immuno-deplete relatively high abundance molecules from the sample to form a first depleted sample. These steps can be repeated a reiterative process of immuno-subtraction to produce second and third depleted samples where the relative abundance of the “low abundance” molecule is increased.
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