Molecule for assigning genotype to phenotype and components thereof as well as method for constructing assigning molecule and method for utilizing assigning molecule
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United States of America Patent
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Oct 2, 2012
Grant Date -
Jan 29, 2004
app pub date -
Jun 13, 2003
filing date -
Dec 14, 2000
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Abstract
An assigning molecule obtained by ligating a genotype molecule to a phenotype molecule by transpeptidation, wherein the genotype molecule is constructed by bonding (a) a spacer molecule comprising a donor region which can be bonded to a 3′-terminal end of nucleic acid, a PEG region bonded to the donor region and a peptide acceptor region which is bonded to the PEG region, to (b) a 3′ end of a nucleic acid comprising a 5′ untranslated region comprising a transcription promoter and a translation enhancer; an ORF region which comprises a polyA sequence and which is bonded to the 3′-terminal side of the 5′ untranslated region; and a 3′-terminal region that is bonded to the 3′-terminal side of the ORF region.
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