Methods for the detection, analysis and isolation of nascent proteins
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United States of America Patent
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Oct 2, 2012
Grant Date -
Oct 13, 2011
app pub date -
Feb 9, 2011
filing date -
Aug 25, 1999
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Abstract
This invention relates to non-radioactive markers that facilitate the detection and analysis of nascent proteins translated within cellular or cell-free translation systems. Nascent proteins containing these markers can be rapidly and efficiently detected, isolated and analyzed without the handling and disposal problems associated with radioactive reagents. Preferred markers are dipyrrometheneboron difluoride (4,4-difluoro-4-bora-3a,4a-diaza-s-indacene)dyes.
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