Modified family 6 glycosidases with altered substrate specificity
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United States of America Patent
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Sep 11, 2012
Grant Date -
Jan 21, 2010
app pub date -
Jul 16, 2009
filing date -
Jul 16, 2008
priority date (Note) -
Expired
status (Latency Note)
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Abstract
A modified Family 6 glycosidase enzyme comprising amino acid substitutions at one or more positions selected from the group 182, 367, 399, 400 and 427 is provided (the position determined form alignment of a parental Family 6 glycosidase with SEQ ID NO: 1). Genetic constructs and genetically modified microbes comprising nucleic sequences encoding the modified Family 6 glycosidase are also provided. Family 6 glycosidase of the invention display decreased hydrolysis activity of beta 1-4 linked polysaccharides and increased hydrolysis activity of beta 1-3, 1-4 linked polysaccharides compared with a parental Family 6 glycosidase. Such glycosidases find use in a variety of applications in industry, e.g., in hydrolysis of beta 1-3, 1-4 linked polysaccharides during the processing of cereal grains or the production of alcohol, animal feed or food products.
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International Classification(s)
- C12C:BREWING OF BEER
- C07H:SUGARS; DERIVATIVES THEREOF; NUCLEOSIDES; NUCLEOTI....
- C12P:FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESI....
- C12N:MICRO-ORGANISMS OR ENZYMES; COMPOSITIONS THEREOF
- A21D:TREATMENT, e.g. PRESERVATION, OF FLOUR OR DOUGH FO....
- C07K:PEPTIDES
- A23B:PRESERVING, e.g. BY CANNING, MEAT, FISH, EGGS, FRU....
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