Method for detection of HCV at the real time PCR with intercalating dye
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United States of America Patent
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Sep 4, 2012
Grant Date -
Jul 8, 2010
app pub date -
Dec 29, 2009
filing date -
Dec 29, 2008
priority date (Note) -
Expired
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Abstract
The present invention relates to a composition for detection of HCV by a single step reaction, comprising a specific primer and probe. In particular, the present invention relates to a composition for detection of HCV by a single step reaction, comprising the primer sequences of SEQ ID NOs:1 and 2; a composition for detection of HCV by a single step reaction, comprising both the primer sequences and a probe of SEQ ID NOs:5 or 9; a method for detecting HCV by a single step reaction, comprising the steps of obtaining a sample from a subject, and amplifying and detecting HCV using the primer and probe; and a kit comprising the primer and probe, in which the HCV detection method is characterized by a single step reaction.
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