Electrotransfer of nucleic acid into tissue cells
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United States of America Patent
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Jul 24, 2012
Grant Date -
Jan 31, 2008
app pub date -
Sep 1, 2006
filing date -
Sep 2, 2005
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Abstract
The electrotransfer of a nucleic acid into tissue cells, in particular in a muscle or a tumoral tissue, is carried out by an electric stimulation of the tissue as follows:
- first with at least one, preferably a single, pulse of a High Voltage field strength of between 200 and 2000 volts/cmsecond with a single pulse of Low Voltage field strength of between 50 and 200 volts/cm and of duration of between 300 ms and 2000 ms.
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