Method for lowering both sequence variations and increase of base line effects in a diagnostic hybridisation assay, assay for performing such a method and probe for use in the assay
Number of patents in Portfolio can not be more than 2000
United States of America Patent
Stats
-
Jul 17, 2012
Grant Date -
Sep 28, 2006
app pub date -
Dec 2, 2003
filing date -
Dec 3, 2002
priority date (Note) -
In Force
status (Latency Note)
Importance
Abstract
The present invention relates to the use in a diagnostic hybridization assay of a molecular beacon probe for lowering: the effect of sequence variations in a nucleic acid analyte, and/or the IBL effect due to the possible opening of the stem-loop structure of a molecular beacon by way of (contaminants in the amplification) enzymes, which assay comprises the steps of contacting a set of primers and a sample containing the nucleic acid analyte to amplify the analyte and detecting the amplified analyte or its complement using a molecular beacon probe, wherein the molecular beacon probe comprises one or more nucleotides and/or nucleotide analogues that have an affinity increasing modification. The invention also relates to such molecular beacon probe and to a kit for performing a diagnostic assay using such molecular beacon probe.
First Claim
Family
International Classification(s)
- [Classification Symbol]
- [Patents Count]
Cited Art Landscape
Patent Citation Ranking
Recipient Email Address
Recipient Email Address
Comment
Recipient Email Address
Add to Portfolio(s)
To add this patent to one, or more, of your portfolios, simply click the add button.
This Patent is in these Portfolios:
Add to additional portfolios:
Last Refreshed On:
Changes done successfully