Methods of separating biopolymer conjugated molecules from unconjugated molecules
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United States of America Patent
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Jun 26, 2012
Grant Date -
Nov 19, 2009
app pub date -
May 1, 2009
filing date -
May 1, 2008
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Abstract
The invention relates to methods for separating or purifying biopolymer conjugated molecules from unconjugated molecules. In particular, methods are described for purifying a PEGylated protein or oligonucleotide from an unPEGylated protein or oligonucleotide, respectively. The methods are quick and efficient separation methods because they do not require gradient chromatography, fractionation of an eluent or analysis of the eluted fractions. Further, the methods increase yield and purity of the biopolymer conjugated molecule.
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