Enzyme compositions and methods for the improved enzymatic hydrolysis of cellulose
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United States of America Patent
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Jun 19, 2012
Grant Date -
Mar 18, 2010
app pub date -
Jun 22, 2007
filing date -
Jun 22, 2006
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Expired
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Abstract
A process for the enzymatic hydrolysis of cellulose to produce a hydrolysis product comprising glucose from a pretreated lignocellulosic feedstock and enzymes for use in the process are provided. The process comprises partially hydrolyzing an aqueous slurry of a pretreated lignocellulosic feedstock with cellulase enzymes, one or more than one β-glucosidase enzyme and a binding agent for binding the β-glucosidase enzyme to fiber solids present in the aqueous slurry. The unhydrolyzed fiber solids are then separated from the hydrolyzed slurry. The separated fiber solids thus obtained are then re-suspended in an aqueous solution to produce a re-suspended slurry. The hydrolysis is then continued to produce the hydrolysis product comprising glucose.
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