Promoter and plasmid system for genetic engineering
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United States of America Patent
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Jun 5, 2012
Grant Date -
Jul 7, 2005
app pub date -
Apr 22, 2003
filing date -
Apr 22, 2002
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Abstract
This invention provides a series of low-copy number plasmids comprising restriction endonuclease recognition sites useful for cloning at least three different genes or operons, each flanked by a terminator sequence, the plasmids containing variants of glucose isomerase promoters for varying levels of protein expression. The materials and methods are useful for genetic engineering in microorganisms, especially where multiple genetic insertions are sought.
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