Covalent joining of DNA strands to RNA strands catalyzed by vaccinia topoisomerase
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United States of America Patent
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Jun 5, 2012
Grant Date -
Dec 16, 2010
app pub date -
Feb 12, 2010
filing date -
Jun 12, 1997
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Abstract
The present invention provides a method of covalently joining a DNA strand to an RNA strand, a method of tagging a 5′ end of an RNA molecule, a DNA-RNA molecule which has been joined in vitro by the use of a topoisomerase, a method of tagging a 5′ end of an mRNA, and a method of isolating and cloning full-length gene sequences using capped mRNA after subtraction of non-capped RNA.
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