Methods and apparatuses for convective polymerase chain reaction (PCR)
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United States of America Patent
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May 29, 2012
Grant Date -
Aug 13, 2009
app pub date -
Jan 26, 2009
filing date -
Jan 24, 2008
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Abstract
The present invention provides a method and apparatus for amplifying a nucleic acid sequence by polymerase chain reaction (PCR). The method comprises placing a PCR sample in a container which is heated by only a single heat source that provides a high temperature for denaturation in the bottom of the PCR sample, while annealing and extension automatically occur in different regions of the PCR sample due to the convection induced by a temperature gradient descending from the bottom of the PCR sample to the surface of the PCR sample.
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