Recombinant North American type 1 porcine reproductive and respiratory syndrome virus and methods of use
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United States of America Patent
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May 22, 2012
Grant Date -
Jun 3, 2010
app pub date -
Jun 24, 2008
filing date -
Jun 25, 2007
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Abstract
Porcine reproductive and respiratory syndrome virus (PRRSV) is a major problem in the pork industry worldwide. The inclusion of markers in vaccines will allow for diagnostic differentiation of vaccinated animals from those naturally infected with wild-type virus. Using a cDNA infectious clone of North American Type 1 PRRSV, a recombinant green fluorescent protein (GFP) tagged PRRSV has been made, containing deletion of an immunogenic epitope, ES4, in the nsp2 region. GFP and ES4 epitope-based ELISAs compliment the marker identification.
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