Process for the preparation of poly(alkoxylated) oligonucleotides
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United States of America Patent
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Apr 24, 2012
Grant Date -
Feb 4, 2010
app pub date -
Nov 20, 2006
filing date -
Dec 7, 2005
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Expired
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Abstract
A process for the preparation of a poly(alkoxylated) oligonucleotide is provided. The process comprises reacting an oligonucleotide which has been purified by ultrafiltration with a poly(alkoxide) thereby to form a poly(alkoxylated) oligonucleotide. The poly(alkoxyalted) oligonucleotide may be separated from non-poly(alkoxyalted) oligonucleotide by ultrafiltration under denaturing conditions, such as the presence of organic solvents, for example, ethanol; the presence of urea; the addition of chaotropic salts, for example perchlorate and guanidinium salts; the presence of formamide; and the application of heat, for example a temperature of up to about 70° C.
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