High production system for infectious hepatitis C virus particle
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United States of America Patent
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Mar 27, 2012
Grant Date -
Feb 11, 2010
app pub date -
Sep 29, 2006
filing date -
Sep 30, 2005
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Expired
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Abstract
The present invention relates to a method for producing infectious hepatitis C virus (HCV) particles, comprising a step of introducing an expression vector into a cell that allows HCV proliferation, such expression vector comprising: DNA sequences encoding the 5′ untranslated region, structural proteins, and, if necessary, non-structural proteins of HCV and DNA sequences encoding non-structural proteins and the 3′ untranslated region derived from the HCV JFH1 strain, which are located downstream of a polymerase I promoter; and a DNA fragment containing an RNA polymerase I terminator, which is located further downstream thereof.
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