Method of assaying target substance in sample, aptamer molecule method of constructing the same
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United States of America Patent
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Mar 27, 2012
Grant Date -
Jan 28, 2010
app pub date -
Sep 27, 2007
filing date -
Sep 27, 2006
priority date (Note) -
Expired
status (Latency Note)
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Abstract
A method for measuring a test substance whereby the test substance in a sample can be specifically measured without using an antibody against the test substance; an aptamer molecule used therefor; and a method for creating the aptamer are disclosed. An aptamer capable of hybridizing with an oligonucleotide when it is bound to a test substance, but is incapable of hybridizing with the oligonucleotide when it is not bound to the test substance, is utilized. The aptamer is brought into contact with a sample, and the aptamer bound to the test substance is brought into contact with an immobilized oligonucleotide which hybridizes with the aptamer, to bind the aptamer to a solid phase, followed by measurement of the aptamer immobilized on the solid phase.
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