Closed-system multi-stage nucleic acid amplification reactions
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United States of America Patent
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Mar 13, 2012
Grant Date -
Aug 10, 2006
app pub date -
Oct 27, 2005
filing date -
Oct 27, 2004
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Abstract
The invention is directed to systems, methods, and apparatus for carrying out multi-stage amplification reactions, especially under fluidly closed conditions. In one aspect, methods of the invention are carried out in a fluidly closed reaction system that permits the isolation of a portion of a first (or prior) reaction mixture and its use as a sample or specimen in a second (or subsequent) reaction mixture, thereby substantially avoiding interfering effects that first reaction components may have in the second reaction if both reaction mixtures were simply combined together. In this aspect, systems, methods, and apparatus of the invention may be used with any amplification reaction that permits multiple stages of amplification based on the use of nested primers.
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