Flow paths comprising one or two porous beds
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United States of America Patent
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Mar 13, 2012
Grant Date -
Oct 18, 2007
app pub date -
Jan 31, 2005
filing date -
Jan 29, 2004
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Abstract
A microfluidic device that comprises a microchannel structure in which there are one, two or more flow paths (101;201a,b;301a,a′,b) all of which comprises a porous bed I (104,204,304) that is common for all of the flow paths and exposes an immobilized reactant R that is capable of interacting with a solute S that passes through the bed. The characteristics are that at least one of the flow paths comprises/comprise a second porous bed II (105,205,305) that is placed upstream of porous bed I (104,204,304) and is dummy with respect to interaction with solute S but capable of interacting with a substance DS that is present in a liquid aliquot together with solute S and is capable of disturbing the result of the interaction between solute S and said immobilized reactant R. There is also disclosed a method utilizing the device and variant of the device in which the immobilized R is replaced with a generic affinity ligand LI and/or porous bed II exposes a generic ligand LII that may be different from LI.
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