Fermentation process starting from cellulosic biomass and involving the recirculation of detoxified stillage into the process
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United States of America Patent
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Feb 7, 2012
Grant Date -
Sep 8, 2011
app pub date -
Sep 30, 2009
filing date -
Sep 30, 2008
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Abstract
A method for preparing a target chemical derivable from cellulosic biomass, involving detoxification of spent hydrolysate is provided. The method comprises the steps of providing cellulosic biomass, subjecting the cellulosic biomass to aqueous pretreatment, aqueous hydrolysis, and fermentation under conditions in which at least a part of the fermentable sugars are fermented into a primary target chemical, separating the primary target chemical from the fermented hydrolysate to provide a spent hydrolysate comprising inhibitory substances and detoxifying the spent hydrolysate by decreasing the concentration of at least one of the inhibitory substances using a detoxification biocatalyst selected from the group consisting of wild type, mutant and recombinant filamentous fungi and recirculating at least a part of the detoxified spent hydrolysate, optionally after further purification.
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