Method for detecting and amplifying nucleic acid
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United States of America Patent
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Jan 17, 2012
Grant Date -
Feb 12, 2009
app pub date -
Nov 21, 2006
filing date -
Nov 25, 2005
priority date (Note) -
Expired
status (Latency Note)
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Abstract
Problem to be solved There is provided a method for detecting and/or amplifying a nucleic acid contained in a biological sample such as blood or cells conveniently, rapidly, and effectively.Solution There is provided a method for detecting a nucleic acid contained in a sample, comprising the step of adding at least one substance selected from the group consisting of polyphenols, polyhydric alcohols, sugar acids, sugar alcohols, and hydrophilic biodegradable polymers to a sample, the step of complementarily binding an oligonucleotide complementary to a part of the nucleic acid sequence of a nucleic acid to be detected to a part of the nucleic acid sequence, and the step of detecting the nucleic acid to be detected.
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