Time-domain method and apparatus for determining the depth and concentration of a fluorophore in a turbid medium
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United States of America Patent
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Dec 27, 2011
Grant Date -
Jun 9, 2011
app pub date -
Feb 9, 2011
filing date -
Oct 31, 2003
priority date (Note) -
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Abstract
Methods and apparatuses for determining the depth and concentration of fluorophores in a turbid medium are disclosed. The method advantageously provides for a rapid estimation of the depth of the flurophore using characteristics of a temporal point spread function. The concentration of the flurophore can be determined using the method of the present invention by combining a calculated depth of the flurophore with a measurement of the intensity of the emitted fluorescence. The intensity can be accurately measured by the apparatus disclosed herein which combine back-reflection and trans-illumination geometries for the source of light injecting and detection.
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