Nucleic acid amplification primers for detecting cytokeratins and examination method with the use of the primers
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United States of America Patent
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Dec 13, 2011
Grant Date -
May 4, 2006
app pub date -
May 20, 2003
filing date -
May 21, 2002
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Abstract
It is intended to provide novel primers to be used in gene amplification reactions for detecting human cytokeratins (Human CK). A primer containing an oligonucleotide comprising a base sequence which is selected from among the the 270- to 1375-th regions of the base sequence represented by SEQ ID NO:1 and the regions of complementary strands thereof and represented by, for example, any of SEQ ID NOS:2 to 341 is constructed. Further, a primer containing an oligonucleotide comprising a base sequence which is selected from among the 270- to 930-th regions of the base sequence represented by SEQ ID NO:342 and the regions of complementary strands thereof and represented by, for example, any of SEQ ID NOS:343 to 434 is constructed. Furthermore, a primer containing an oligonucleotide comprising a base sequence which is selected from among the 340 to 490-th regions or the 495- to 1050-th regions of the base sequence represented by SEQ ID NO:435 and the regions of complementary strands thereof and represented by, for example, any of SEQ ID NOS:436 to 474 is constructed.
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