Isolation of DNA, RNA and protein from a single sample
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United States of America Patent
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Nov 29, 2011
Grant Date -
Nov 18, 2010
app pub date -
Feb 5, 2009
filing date -
Feb 7, 2008
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Abstract
The invention provides a method for the separation and purification of two or three cellular components selected from genomic DNA, RNA and proteins from a single biological sample. The method comprises generating an aqueous solution containing the cellular components by lysing cells with a lysis solution; contacting the aqueous solution with an ion exchanger for genomic DNA and RNA to bind to the ion exchanger; collecting the flow-through which contains unbound proteins; eluting RNA from the ion exchanger; and eluting DNA from the ion exchanger. For the purification of any two of the cellular components, one of the components is not collected. The invention also provides reagent kits for carrying out the methods.
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