Polymerase chain reaction (PCR) method for amplifying a DNA template
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United States of America Patent
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Nov 29, 2011
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N/A
app pub date -
May 30, 2007
filing date -
Oct 4, 2000
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Abstract
The present invention relates to a DNA polymerase immobilized by covalent bonding. More particularly, the present invention relates to an immobilized DNA polymerase whose activity is maximally preserved by masking the active site of the DNA polymerase and optimizing interaction of the masked molecule to the substrate material. In one embodiment, the average activity of the immobilized DNA polymerase is more than about 10% relative to that of the solution phase DNA polymerase. Further provided by the invention are methods and kits for performing polymerase chain reactions (PCR).
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