Diagnostic histopathology using multiplex gene expression FISH
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United States of America Patent
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Nov 22, 2011
Grant Date -
Sep 7, 2006
app pub date -
Apr 14, 2006
filing date -
Jul 21, 2003
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Abstract
The present invention relates to a method for detecting one or more nascent RNAs in a tissue sample using FISH. In the method, a plurality of probes (8 to 82) may be used to detect a single species of nascent RNA. Further, a plurality of nascent RNA species may be detected simultaneously using between 8 and 82 probes for each nascent RNA. The invention comprises, in addition, methods of preparing a sample for nascent RNA detection by reducing autofluorescence of the tissue sample. These techniques may be synergistically combined to achieve significantly improved results.
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