Unnatural polymerase substrates that can sustain enzymatic synthesis of double stranded nucleic acids from a nucleic acid template and methods of use

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United States of America Patent

PATENT NO 8058414
APP PUB NO 20090269759A1
SERIAL NO

12355487

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Abstract

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Nucleotide analogs that can sustain the enzymatic synthesis of double-stranded nucleic acid from a nucleic template are described. The nucleotide analogs include: (i) a base selected from the group consisting of adenine, guanine, cytosine, thymine, uracil and their analogs; (ii) a label attached to the base or analog of the base via a cleavable linker; (iii) a deoxyribose; and (iv) one or more phosphate groups. The linker and/or the label inhibits template directed polymerase incorporation of a further nucleotide substrate onto an extended primer strand. In addition, cleavage of the linker leaves a residue attached to the base which is not present in the natural nucleotide and which does not inhibit extension of the primer strand. The nucleotide analogs can therefore be used as reversible terminators in sequencing by synthesis methods without blocking the 3′ hydroxyl group. Methods of sequencing DNA using the substrates are also described.

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Patent Owner(s)

Patent OwnerAddress
LIFE TECHNOLOGIES5791 VAN ALLEN WAY CARLSBAD CA 92008

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Inventor(s)

Inventor Name Address # of filed Patents Total Citations
Chen, Jer-Kang Palo Alto, US 22 1611
Huang, Boli Palo Alto, US 6 95
Kenney, Paul Sunnyvale, US 14 162
Khan, Shoeb Foster City, US 2 49
Lam, Joe Y Castro Valley, US 6 91
Ma, Zhaochun Sunnyvale, US 27 366
Menchen, Steven Fremont, US 42 322
Rosenblum, Barnett San Jose, US 35 1123

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