Method for making polyalkylene oxide modified hemoglobin
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United States of America Patent
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Sep 20, 2011
Grant Date -
Mar 25, 2010
app pub date -
Aug 6, 2009
filing date -
Sep 22, 2008
priority date (Note) -
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Abstract
The present invention is a method for preparing a hemoglobin based oxygen carrier to deliver oxygen to tissue. The method comprises mixing a deoxygenated hemoglobin with a maleimide-activated polyakylene oxide (PAO) to obtain an oxygen carrying product. This product is characterized as having 2 to about 8 PAO moieties covalently bound to a deoxygenated hemoglobin and a p50 less than native stroma-free hemoglobin from the same animal source when measured under the same conditions.
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