Method of amplifying nucleic acid by using double-stranded nucleic acid as template
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United States of America Patent
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Sep 13, 2011
Grant Date -
Nov 6, 2003
app pub date -
Mar 30, 2001
filing date -
Apr 7, 2000
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Abstract
The present invention provides a nucleic acid synthesis method which involves the step of incubating a double-stranded nucleic acid template under conditions that ensure a complementary strand synthesis reaction using a primer as an origin. This method involves the step of placing a region, to which a primer capable of isothermally amplifying the template nucleic acid will anneal, in a condition that ensures base pairing, using an arbitrary primer. The arbitrary primer initiates the complementary strand synthesis reaction, using the double-stranded nucleic acid as a template and DNA polymerases catalyzing the complementary strand synthesis reaction which comprises the destabilization of the double-stranded nucleic acid and strand displacement, thereby providing a region that can undergo base pairing.
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