Trophic conversion of obligate phototrophic algae through metabolic engineering
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United States of America Patent
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Aug 30, 2011
Grant Date -
Jun 12, 2008
app pub date -
Aug 21, 2007
filing date -
Apr 21, 2000
priority date (Note) -
Expired
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Abstract
, allowing the cells to metabolize exogenous organic carbon and thrive, independent of light. This is the first successful trophic conversion of an obligate photoautotroph through metabolic engineering, and it demonstrates that methods of cell nourishment can be fundamentally altered with the introduction of a single gene. Since strains transformed with the glucose transport genes are able to grow non-photosynthetically, they can be exploited for the analysis of photosynthetic processes through mutant generation and characterization. Finally, this work also represents critical progress toward large-scale commercial exploitation of obligate phototrophic algae through the use of microbial fermentation technology, eliminating significant limitations resulting from light-dependent growth.
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