Method utilizing enzyme substrates producing insoluble fluorescent products and chromogens, and use in combination with enzyme substrates producing soluble products
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United States of America Patent
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Aug 30, 2011
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Mar 16, 2007
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Mar 17, 2006
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Abstract
A method of detection of cells, microorganisms, or molecules by the use of various combinations of fluorogens and a chromogens which yield fluorophores and chromophores when cleaved by specific enzymes and which can be viewed by UV and visible light. Included is the method of application of a family of compounds producing both insoluble fluorophores and chromophores identified as dual enzyme substrates.
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