Mutant PCNA
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United States of America Patent
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Aug 23, 2011
Grant Date -
Aug 20, 2009
app pub date -
Jul 4, 2006
filing date -
Jul 4, 2005
priority date (Note) -
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Abstract
The present invention is to construct a DNA replication reaction system which is excellent in versatility and is easily used. An amino acid sequence of a PCNA monomer which is one of factors involved in DNA replication is prepared so that amino acid residues causing mutual charge repulsion constitute a site which causes, when an N terminal region of the PCNA monomer and a C terminal region of another PCNA monomer act as an interface to form a multimeric complex, an intermolecular interaction of the monomers in an interface region of the monomers.
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