Method for determining diamine oxidase
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United States of America Patent
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Aug 23, 2011
Grant Date -
Sep 18, 2008
app pub date -
Nov 17, 2006
filing date -
Nov 18, 2005
priority date (Note) -
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Abstract
Disclosed is a method for determining histaminase (DAO; EC 1.4.3.6) activity in a sample. Said method comprises the following steps: an aqueous solution is supplied which contains a predefined amount of a diamine; the aqueous solution is mixed and incubated with the sample for a given period of time in conditions in which the diamine can be reacted with a DAO possibly present in the sample; the diamine is derivatized; the amount of derivatized diamine is determined; the predefined amount of diamine is compared to the amount of derivatized diamine; and the activity of histaminase possibly present is determined.
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